Food Technology and Nutrition Center of Excellence
The Minifors 2 is a compact and easy-to-use bioreactor with a full range of applications. It allows easy and continuous culture of microorganisms such as Escherichia coli, Saccheromyces cerevisiae and various Lactobacilli. Fermentation can be carried out under oxygen supply (aerobic) or in the absence of air (anaerobic). The biotic raw material is modified by microbial as well as autolytic enzymatic processes.
Technical Data: 3.0 L total volume with rounded, flat bottom | stirrer speed from 150 to 1600 min-1 | two 6-blade disc stirrers (Rushton) | 2 integrated mass flow controllers for air, air/O2 or air/N2 (for culture of microorganisms) | temperature control via exchangeable heating/cooling block | acquisition of online parameters
Microbial profiling using 16S ribosomal RNA (rRNA) sequencing is a common method for studying bacterial phylogeny and taxonomy. The 16S rRNA gene is the most established genetic marker used for bacterial identification and classification, primarily because it consists of both highly conserved and hypervariable regions. The conserved regions can serve as universal primer binding sites for amplification of the entire gene or fragments of the gene, while the hypervariable regions contain species-specific sequences that can distinguish between different bacteria and archaea. Similarly, the internal transcribed spacer (ITS) region is used for taxonomic profiling of fungi.
Microbiome Sequencing in cooperation with corporate partners.